Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Por um escritor misterioso
Descrição
Advances in RNA fluorescence in situ hybridization (RNA FISH) have allowed practitioners to detect individual RNA molecules in single cells via fluorescence microscopy, enabling highly accurate and sensitive quantification of gene expression. However, current methods typically employ hybridization times on the order of 2–16 hours, limiting its potential in applications like rapid diagnostics. We present here a set of conditions for RNA FISH (dubbed Turbo RNA FISH) that allow us to make accurate measurements with no more than 5 minutes of hybridization time and 3 minutes of washing, and show that hybridization times can go as low as 30 seconds while still producing quantifiable images. We further show that rapid hybridization is compatible with our recently developed iceFISH and SNP FISH variants of RNA FISH that enable chromosome and single base discrimination, respectively. Our method is simple and cost effective, and has the potential to dramatically increase the throughput and realm of applicability of RNA FISH.
Papers — Syd Shaffer Lab
RNA FISH experiment flowchart. The flowchart illustrates the main
Detection of mRNA Transfer Between Mammalian Cells in Coculture by Single- Molecule Fluorescent In Situ Hybridization (smFISH)
Molecules, Free Full-Text
Single-Molecule RNA FISH
Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Design, Labeling, and Application of Probes for RNA smFISH
Genome oligopaint via local denaturation fluorescence in situ hybridization - ScienceDirect
Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response - ScienceDirect
FISHing for Accurate Transcript Counts with Single-Molecule FISH
Using Single Molecule mRNA Fluorescent in Situ Hybridization (RNA-FISH) to Quantify mRNAs in Individual Murine Oocytes and Embryos
Comparison of fixation conditions for both traditional overnight
Stellaris RNA FISH Buffers LGC Biosearch Technologies
de
por adulto (o preço varia de acordo com o tamanho do grupo)
