Casa
scp 7143 j

Transgenic Mice Expressing Green Fluorescent Protein under the

Por um escritor misterioso

Descrição
The melanocortin-4 receptor (MC4-R) is an important regulator of energy homeostasis, and evidence suggests that MC4-R-expressing neurons are downstream targets of leptin action. MC4-Rs are broadly expressed in the CNS, and the distribution of MC4-R mRNA has been analyzed most extensively in the rat. However, relatively little is known concerning chemical profiles of MC4-R-expressing neurons. The extent to which central melanocortins act presynaptically or postsynaptically on MC4-Rs is also unknown. To address these issues, we have generated a transgenic mouse line expressing green fluorescent protein (GFP) under the control of the MC4-R promoter, using a modified bacterial artificial chromosome. We have confirmed that the CNS distribution of GFP-producing cells is identical to that of MC4-R mRNA in wild-type mice and that nearly all GFP-producing cells coexpress MC4-R mRNA. For example, cells coexpressing GFP and MC4-R mRNA were distributed in the paraventricular hypothalamic nucleus (PVH) and the dorsal motor nucleus of the vagus (DMV). MC4-R promotor-driven GFP expression was found in PVH cells producing thyrotropin-releasing hormone and in cholinergic DMV cells. Finally, we have observed that a synthetic MC3/4-R agonist, MT-II, depolarizes some GFP-expressing cells, suggesting that MC4-Rs function postsynaptically in some instances and may function presynaptically in others. These studies extend our knowledge of the distribution and function of the MC4-R. The transgenic mouse line should be useful for future studies on the role of melanocortin signaling in regulating feeding behavior and autonomic homeostasis.
Transgenic Mice Expressing Green Fluorescent Protein under the
Targeted Expression of Mutated ALK Induces Neuroblastoma in
Transgenic Mice Expressing Green Fluorescent Protein under the
Recombinant Green Fluorescent Protein Cat# Prot-r-AcGFP – EnCor
Transgenic Mice Expressing Green Fluorescent Protein under the
Generating green fluorescent mice by germline transmission of
Transgenic Mice Expressing Green Fluorescent Protein under the
Hematopoietic stem cell-specific GFP-expressing transgenic mice
Transgenic Mice Expressing Green Fluorescent Protein under the
GFP Polyclonal Antibody, Alexa Fluor™ 488 (A-21311)
Transgenic Mice Expressing Green Fluorescent Protein under the
Reporter Mouse Lines for Fluorescence Imaging - Abe - 2013
Transgenic Mice Expressing Green Fluorescent Protein under the
GFP mice JCRB Laboratory Animal Resource Bank at NIBIOHN
Transgenic Mice Expressing Green Fluorescent Protein under the
Frontiers Fluorescent transgenic mouse models for whole-brain
Transgenic Mice Expressing Green Fluorescent Protein under the
Anti-Green Fluorescent Protein Antibody
Transgenic Mice Expressing Green Fluorescent Protein under the
Retrograde trans-synaptic transfer of green fluorescent protein
Transgenic Mice Expressing Green Fluorescent Protein under the
Choosing Fluorescent Reagents for Every Live Cell Application
de por adulto (o preço varia de acordo com o tamanho do grupo)